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This line of research is being done by the Urology Disciplines (Sector of Feminine Urology, urodynamic and Neurourology) and Neurology (Sector of Neurophysiology clinic) of the Universidade Federal de São Paulo (UNIFESP) . This work was done on women whose history and clinical evaluation did not evidence the presence of urine loss or feces, significative genital prolapse, diabetes and other degenerative alterations, neurological pathologies, vaginal or extensive pelvic surgeries, urinary infection or suggestive problem of interstitial cystitis.
A total of 9 volunteers were part of this study, after reading the letter of written information and permission, with age between 29-63 (mean=44.4 years).The patient was put in lythotomy position to research both reflexes. We initially got a sensorial threshold raising gradually the intensity of the stimulus until its perception by the patient. Because it is a subjective data successive repetition was necessary till we obtained a trustful value. Subsequent stimulus were done with multiples of the sensitive threshold.
In the pudendal anal reflex stimulation occurred in the paraclitoris region, bilaterally, through a conventional bipolar stimulator. The mid urethra region was stimulated, to obtain a urethro-anal reflex, through ring electrodes DANTEC coupled to a Foley probe 10 Fr. Electrodes on the surface together with the perianal region registered the results in an eletromyography apparatus NIHON-KOHDEN model Neuropack sigma.
Repeated stimulus in each intensity determined by the sensitive threshold were done. The latency obtained in milliseconds (ms) through the superposition of the observed traces were analized.